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Study of the limiting steps in AAV-mediated gene transfer and development of improved vectors.

Units : Laboratory of Experimental Neurosurgery | ULB257

Description :

In the basal ganglia, recombinant vectors for gene transfer based on adeno-associated virus (AAV), a single-standed virus,
transduce neurons of the globus pallidus and substantia nigra with a high efficiency. In contrast, transduction effeciency is low in the
striatum. In order to identify the limiting steps in AAV-mediated gene transfer in the striatum, new vectors are currently
evaluated: I) self-complementary vectors allowing the formation of double-standed DNA without the need for a cellular factor; II)
non-viral vectors consisting in recombinant capsid protein of JCV, a papovavirus with a natural tropism for glial cells in the brain
(collaboration with W. Lüke) and AAV DNA.

List of persons in charge :


List of lessors :

  • F.R.S.-FNRS et Fonds associés (hors FRIA)

  • Fonds associés (toutes subventions, y compris la Loterie Nat.)

  • Autres fonds publics belges

  • Autres U.E.