Ovarian tissue cryopreservation is usually proposed to preserve fertility of patients at risk of premature ovarian failure after
antineoplastic treatment. At present, ovarian tissue transplantation is the only option to restore fertility using cryopreserved
ovarian tissue. However, this procedure has several limitations and the present project aims to develop in vitro follicles culture
systems in order to produce embryos from stored ovarian tissue without graft. The procedure has been achieved in mice but is still
at the beginning in large mammals and in human. In our laboratory, follicular culture system has been largely studied in mice
model and we have now developed research program investigating different aspect of human follicular activation in vitro in order to
support follicle growth from primordial follicles to maturity. Recently, research projects focus on the role of PI3K/Akt/mTOR and
Hippo pathway in the activation of human or animal follicles in vitro.